Chromatofocusing in the purification of staphylococcal enterotoxin D.
نویسندگان
چکیده
A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.
منابع مشابه
Chromatofocusing: a new method for purification of staphylococcal enterotoxins B and C1.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 26 6 شماره
صفحات -
تاریخ انتشار 1988